Evidence for Metabolic Domains within the Matrix Compartment of Pea Leaf Mitochondria
نویسندگان
چکیده
منابع مشابه
Characterization of the transport of oxaloacetate by pea leaf mitochondria.
Mitochondria isolated from pea (Pisum sativum L.) leaves are able to transport the keto acid, oxaloacetate, from the reaction medium into he mitochondrial matrix at high rates. The rate of uptake by the mitochondria was measured as the rate of disappearance of oxaloacetate from the reaction medium as it was reduced by matrix malate dehydrogenase using NADH provided by glycine oxidation. The oxa...
متن کاملThe Regulation of Pyruvate Dehydrogenase Activity in Pea Leaf Mitochondria'
The regulation of the pea (Pisum safivum) leaf mitochondrial pyruvate dehydrogenase complex by respiratory rate and oxidative phosphorylation has been investigated by measuring the respiratory activity, the redox poise of the quinone pool (Q-pool), and mitochondrial pyruvate dehydrogenase (mtPDC) activity under various metabolic conditions. It was found that, under state 4 conditions, mtPDC act...
متن کاملSimultaneous oxidation of glycine and malate by pea leaf mitochondria.
Mitochondria isolated from pea leaves (Pisum sativum L.) readily oxidized malate and glycine as substrates. The addition of glycine to mitochondria oxidizing malate in state 3 diminished the rate of malate oxidation. When glycine was added to mitochondria oxidizing malate in state 4, however, the rate of malate oxidation was either unaffected or stimulated. The reason both glycine and malate ca...
متن کاملGlycine metabolism and oxalacetate transport by pea leaf mitochondria.
Isolated pea leaf mitochondria oxidatively decarboxylate added glycine. This decarboxylation could be linked to the respiratory chain (in which case it was coupled to three phosphorylations) or to mitochondrial malate dehydrogenase when oxalacetate was supplied. Decarboxylation rates measured as O(2) uptake, or CO(2) and NH(3) release were adequate to account for whole leaf photorespiration. Ox...
متن کاملComparison with Translocators from Spinach Chloroplasts and Pea Leaf Mitochondria
The kinetic properties of the adenosine 5’-diphosphate/adenosine 5’-triphosphate (ADP/ATP) translocator from pea (Pisum sativum l.) root plastids were determined by silicone oi l filtering centrifugation and compared with those of spinach (Spinacia oleracea 1.) chloroplasts and pea leaf mitochondria. In addition, the ADP/ATP transporting activities from the above organelles were reconstituted i...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1990
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.93.2.611